Windows 2000/XP/Vista Download (13.2 MB file)
Mac OS X v10.4 or Greater Download (20.0 MB file)

I don't know, could be worth a try?

edit.

http://www.lostmarble.com/forum/viewtopic.php?t=11809&sid=77bf16d2f1443eeeeb28aeef12a3e75d

if you read the third post day they say

"I can tell you that Anime Studio runs fine on Vista 64. It's not a 64 bit process, but otherwise it works just fine"

http://my.smithmicro.com/freeanime/

This isn't the lastest version, which is 6

Product description

Anime Studio includes everything aspiring artists and hobbyists need to create quality animations from start to finish. Anime Studio simplifies the animation process, empowering artists to create their projects faster and easier than ever before.

• Get started quickly - Anime Studio is packed with ready-to-use anime style characters so you can start creating your own animations right away. Tutorials and sample files show you how to maximize Anime Studio’s powerful features giving you the jump needed from your first project onwards.
• Your art, your way - To get started, sketch your original art, scan in or import graphics, or if you use a pen tablet you can recreate the sensation of drawing on paper.
• Vector graphics provide power and flexibility - Use multiple layering and resize your images with no loss in quality.
• Animate your characters using bones - Using Anime Studio’s bone rigging system, you can add a skeleton to any image by point-and-click to bring it to life. With Anime Studio, there’s no need to create new art for each individual frame as you animate. Just adjust the bones to your desired position and the program will generate the movement for you. It’s that easy!
• Direct your action with an intuitive timeline - Setting your movements to a timeline makes animation easy to understand. Anime Studio will create animations up to two minutes long.
• Add audio soundtracks to your animations - Add emotion and style to your animations by adding sound. Anime Studio supports WAV and AIFF formats.
• Integrate with Adobe Photoshop - Import layered PSD files using a Photoshop plug-in that converts files to Anime Studio format, included free with Anime Studio.
• Publish for multiple media - Output your animations to video, TV, and web formats.

Whether you're a digital enthusiast, a newcomer to animation, or if you want to create art for work or fun, Anime Studio provides what you need to create your own animations faster than anything else available!

Windows

• Windows 98 Second Edition, Windows ME, 2000 (Service Pack 2 or higher), Windows XP Home/Professional/Tablet PC Edition
• 500 MHz Intel Pentium or equivalent Processor minimum
• 256 MB RAM minimum
• 100 MB free hard disk space minimum
• 16 bit color (65,536 colors) display or above 1024x768 resolution
• CD-ROM (for physical version of application)

Macintosh

• Mac OS X v10.4+ (Universal Binary)
• PowerPC G5/G4 Processor 500MHz or above (Intel Processor recommended)
• 256 MB RAM minimum
• 100 MB free hard disk space minimum
• 16 bit color (65,536 colors) display or above 1024x768 resolution
• CD-ROM (for physical version of application)

http://shop.smithmicro.com/dr/v2/ec_Main.Entry17c?SID=1839&SP=10023&CID=374740&PID=897044&PN=1&V1=897044&CUR=840&API1=65&API2=GOOGLE&API3=anime_studio_exa&API4=Empty&API5=Empty&DSP=&PGRP=0&ABCODE=&CACHE_ID=374740